Table 1 Characteristics of patients in the included studies and assessment of the risk of bias
From: Human herpesvirus 6B infection in mesial temporal lobe epilepsy: a meta-analysis
Reference | Country | Patients (n) | Age (years) | Febrile convulsions (n) | Selection | Comparability | Exposure | Source of tissue sample | DNA extraction method | RNA extraction method | Real-Time quantitative PCR | |||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
MTLE (M/F) | Non-MTLE (M/F) | MTLE | Non-MTLE | MTLE | Non-MTLE | |||||||||||
Li et al. [5] | China | 19 | 13 | 9 | 3 | 23.21 ± 6.64 | 33.41 ± 10.25 | 11 | 0 | *** | ** | *** | Temporal neocortical and hippocampal tissues | DNeasy tissue kit (Qiagen,Germany) | RNA isolation kit (BS574, Sangon) | DNeasy tissue kit (Qiagen, Germany) |
Donati et al. [8] | USA | 4 | 4 | 5 | 2 | 11.55 + 10.0 | 25.5 ± 21.1 | 3 | 0 | **** | ** | ** | Hippocampus,lateral temporal lobe | DNAeasy tissue kit (Qiagen, Valencia, CA) | TaqMan plate | |
Huang et al. [7] | China | 25 | 21 | 12 | 7 | 14.1 ± 7.2 | 17.3 ± 9.1 | 13 | 0 | **** | * | *** | Temporal neocortical and/or hippocampal tissues | QIAamp DNA Micro kit (Qiagen, Germany) | TaqMan PCR | |
Fotheringham et al. [9] | USA | 16 | 7 | 25.875 ± 12.5 | 16.2 ± 13.3 | 5 | 0 | *** | * | *** | Mesial temporal and neocortical resections | QIAamp blood kit | QIAamp Viral RNA kit | TaqMan PCR | ||
Kawamura et al. [10] | Japan | 21 | 31 | 11 | 12 | 16.0 ± 0.75 | 13 ± 8.75 | 27 | 3 | **** | ** | ** | Hippocampus,amygdala, and amygdala mixed with uncus | RNA-to-cDNA Kit (QIAGEN) | RNAeasy Mini Kit (QIAGEN) | TaqMan Fast Universal PCR Master Mix |
Karatas et al. [6] | USA | 15 | 18 | 3 | 4 | 24.7 ± 6.37 | 26.14 ± 7.03 | 3 | 0 | *** | ** | *** | Temporal neocortical and hippocampal tissues | QIAamp blood kit | TaqMan PCR | |