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Fig. 4 | Acta Epileptologica

Fig. 4

From: Long non-coding RNA Tug1 regulates inflammation in microglia and in status epilepticus rats through the NF-κB signaling pathway

Fig. 4

Effect of Tug1 overexpression on LPS-induced inflammation in microglia. a Rat microglia infected with green fluorescence-labeled adenovirus. b Transfection efficiency of lncRNA Tug1 overexpression adenovirus at different multiplicities of infection. c Overexpression of lncRNA Tug1 in microglia. d The level of M1-associated markers mRNA in microglia cells. e The mRNA of M2-associated markers CD163 and Arginase-1 in rat microglia. f Protein bands of p-p65, p65, p-ΙκΒα and IκΒα in rat microglia. g-h The relative level of p-p65/p65 and p-ΙκΒα/ΙκΒα. i Protein bands of IL-1β and TNF-α in rat microglia. j-k The relative protein level of IL-1β and TNF-α. (*P < 0.05, **P < 0.01 vs. Control; #P < 0.05, ##P < 0.01 vs. LPS + OE-NC)

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