Fig. 3
NSE and S100β levels are not associated with time-of-day or age of subject. a Each NSE measurement for all EMU and CRU subjects was converted to percent of maximum for the individual and plotted against time-of-day (CRU samples are shown on day 2 in order to capture the entire 6 AM to 6 PM scale). There is no apparent pattern in the distribution of maximum or minimum NSE levels, suggesting that time-of-day did not drive the variations observed in the EMU patients. b Absolute NSE values measured in 40 single-draw healthy controls were plotted against age at time of blood collection, revealing no relationship (R2 = 0.002). c S100β levels in 34 single-draw healthy control subjects were also not correlated with age (R2 = 0.07). d The median NSE level for each individual EMU (red) and CRU (green) subject was also plotted against age and overlaid with the single sample healthy control values (blue). Again, no apparent relationship between age and serum NSE was revealed. e Similarly, no relationship between median serum S100β and age was apparent in EMU or CRU subjects